Injectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells
Identifieur interne : 002422 ( Main/Exploration ); précédent : 002421; suivant : 002423Injectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells
Auteurs : Yoichi Yamada [Japon, États-Unis] ; Sayaka Nakamura [États-Unis, Japon] ; Kenji Ito [Japon] ; Eri Umemura [Japon] ; Kenji Hara [Japon] ; Tetsuro Nagasaka [Japon] ; Akihiro Abe [Japon] ; Shunsuke Baba [Japon] ; Yasushi Furuichi [Japon] ; Yuichi Izumi [Japon] ; Ophir D. Klein [États-Unis] ; Toshihiko Wakabayashi [Japon]Source :
- Stem cells (Dayton, Ohio) [ 1066-5099 ] ; 2013.
Abstract
Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, very few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectable tissue-engineered bone composed of mesenchymal stem cells (MSCs) and platelet rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out pilot trial cases in patients with long-term follow up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months was significantly higher than the pre-operation baseline (
Url:
DOI: 10.1002/stem.1300
PubMed: 23225744
PubMed Central: 4043220
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Injectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells</title><author><name sortKey="Yamada, Yoichi" sort="Yamada, Yoichi" uniqKey="Yamada Y" first="Yoichi" last="Yamada">Yoichi Yamada</name><affiliation wicri:level="1"><nlm:aff id="A1">Center for Genetic and Regenerative Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Center for Genetic and Regenerative Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">Department of Oral and Maxillofacial Surgery, Aichi Medical University School of Medicine, Yazako, Nagakute, Aichi, 480-1195, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Aichi Medical University School of Medicine, Yazako, Nagakute, Aichi, 480-1195</wicri:regionArea><wicri:noRegion>480-1195</wicri:noRegion></affiliation></author><author><name sortKey="Nakamura, Sayaka" sort="Nakamura, Sayaka" uniqKey="Nakamura S" first="Sayaka" last="Nakamura">Sayaka Nakamura</name><affiliation wicri:level="2"><nlm:aff id="A2">Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Ito, Kenji" sort="Ito, Kenji" uniqKey="Ito K" first="Kenji" last="Ito">Kenji Ito</name><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Umemura, Eri" sort="Umemura, Eri" uniqKey="Umemura E" first="Eri" last="Umemura">Eri Umemura</name><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Hara, Kenji" sort="Hara, Kenji" uniqKey="Hara K" first="Kenji" last="Hara">Kenji Hara</name><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Nagasaka, Tetsuro" sort="Nagasaka, Tetsuro" uniqKey="Nagasaka T" first="Tetsuro" last="Nagasaka">Tetsuro Nagasaka</name><affiliation wicri:level="1"><nlm:aff id="A5">Department of Medical Technology, School of Health Science, Nagoya University, 1-1-20 Daikominami, Higashi-ku, Nagoya, 461-8673, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Medical Technology, School of Health Science, Nagoya University, 1-1-20 Daikominami, Higashi-ku, Nagoya, 461-8673</wicri:regionArea><wicri:noRegion>461-8673</wicri:noRegion></affiliation></author><author><name sortKey="Abe, Akihiro" sort="Abe, Akihiro" uniqKey="Abe A" first="Akihiro" last="Abe">Akihiro Abe</name><affiliation wicri:level="1"><nlm:aff id="A6">Department of Hematology, Fujita Health University, 1–98. Dengakugakubo, Kutsukake-cho,Toyoake, Aichi 470–1192, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Hematology, Fujita Health University, 1–98. 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sortKey="Yamada, Yoichi" sort="Yamada, Yoichi" uniqKey="Yamada Y" first="Yoichi" last="Yamada">Yoichi Yamada</name><affiliation wicri:level="1"><nlm:aff id="A1">Center for Genetic and Regenerative Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Center for Genetic and Regenerative Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">Department of Oral and Maxillofacial Surgery, Aichi Medical University School of Medicine, Yazako, Nagakute, Aichi, 480-1195, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Aichi Medical University School of Medicine, Yazako, Nagakute, Aichi, 480-1195</wicri:regionArea><wicri:noRegion>480-1195</wicri:noRegion></affiliation></author><author><name sortKey="Nakamura, Sayaka" sort="Nakamura, Sayaka" uniqKey="Nakamura S" first="Sayaka" last="Nakamura">Sayaka Nakamura</name><affiliation wicri:level="2"><nlm:aff id="A2">Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Ito, Kenji" sort="Ito, Kenji" uniqKey="Ito K" first="Kenji" last="Ito">Kenji Ito</name><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Umemura, Eri" sort="Umemura, Eri" uniqKey="Umemura E" first="Eri" last="Umemura">Eri Umemura</name><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Hara, Kenji" sort="Hara, Kenji" uniqKey="Hara K" first="Kenji" last="Hara">Kenji Hara</name><affiliation wicri:level="1"><nlm:aff id="A4">Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author><author><name sortKey="Nagasaka, Tetsuro" sort="Nagasaka, Tetsuro" uniqKey="Nagasaka T" first="Tetsuro" last="Nagasaka">Tetsuro Nagasaka</name><affiliation wicri:level="1"><nlm:aff id="A5">Department of Medical Technology, School of Health Science, Nagoya University, 1-1-20 Daikominami, Higashi-ku, Nagoya, 461-8673, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Medical Technology, School of Health Science, Nagoya University, 1-1-20 Daikominami, Higashi-ku, Nagoya, 461-8673</wicri:regionArea><wicri:noRegion>461-8673</wicri:noRegion></affiliation></author><author><name sortKey="Abe, Akihiro" sort="Abe, Akihiro" uniqKey="Abe A" first="Akihiro" last="Abe">Akihiro Abe</name><affiliation wicri:level="1"><nlm:aff id="A6">Department of Hematology, Fujita Health University, 1–98. Dengakugakubo, Kutsukake-cho,Toyoake, Aichi 470–1192, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Hematology, Fujita Health University, 1–98. Dengakugakubo, Kutsukake-cho,Toyoake, Aichi 470–1192</wicri:regionArea><wicri:noRegion>Aichi 470–1192</wicri:noRegion></affiliation></author><author><name sortKey="Baba, Shunsuke" sort="Baba, Shunsuke" uniqKey="Baba S" first="Shunsuke" last="Baba">Shunsuke Baba</name><affiliation wicri:level="1"><nlm:aff id="A7">Department of Oral Implantology, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata, Osaka 573-1121, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Oral Implantology, Osaka Dental University, 8-1 Kuzuhahanazono-cho Hirakata, Osaka 573-1121</wicri:regionArea><wicri:noRegion>Osaka 573-1121</wicri:noRegion></affiliation></author><author><name sortKey="Furuichi, Yasushi" sort="Furuichi, Yasushi" uniqKey="Furuichi Y" first="Yasushi" last="Furuichi">Yasushi Furuichi</name><affiliation wicri:level="1"><nlm:aff id="A8">Division of Periodontology & Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Periodontology & Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293</wicri:regionArea><wicri:noRegion>061-0293</wicri:noRegion></affiliation></author><author><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><affiliation wicri:level="1"><nlm:aff id="A9">Department of Periodontology, Graduate school of Medical and Dental Sciences Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Periodontology, Graduate school of Medical and Dental Sciences Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Klein, Ophir D" sort="Klein, Ophir D" uniqKey="Klein O" first="Ophir D." last="Klein">Ophir D. Klein</name><affiliation wicri:level="2"><nlm:aff id="A2">Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143, USA.</nlm:aff><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Departments of Orofacial Sciences and Pediatrics and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, CA 94143</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Wakabayashi, Toshihiko" sort="Wakabayashi, Toshihiko" uniqKey="Wakabayashi T" first="Toshihiko" last="Wakabayashi">Toshihiko Wakabayashi</name><affiliation wicri:level="1"><nlm:aff id="A1">Center for Genetic and Regenerative Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Center for Genetic and Regenerative Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A10">Department of Neurosurgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Department of Neurosurgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550</wicri:regionArea><wicri:noRegion>Nagoya 466-8550</wicri:noRegion></affiliation></author></analytic><series><title level="j">Stem cells (Dayton, Ohio)</title><idno type="ISSN">1066-5099</idno><idno type="eISSN">1549-4918</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, very few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectable tissue-engineered bone composed of mesenchymal stem cells (MSCs) and platelet rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out pilot trial cases in patients with long-term follow up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months was significantly higher than the pre-operation baseline (<italic>P</italic> <0.001) and reached levels equivalent to that of native bone. No significant bone resorption occurred during long term follow-up. Injectable tissue-engineered bone restored masticatory function in patients. This novel clinical approach represents an effective therapeutic utilization of bone tissue engineering.</p></div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country><region><li>Californie</li><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><noRegion><name sortKey="Yamada, Yoichi" sort="Yamada, Yoichi" uniqKey="Yamada Y" first="Yoichi" last="Yamada">Yoichi Yamada</name></noRegion><name sortKey="Abe, Akihiro" sort="Abe, Akihiro" uniqKey="Abe A" first="Akihiro" last="Abe">Akihiro Abe</name><name sortKey="Baba, Shunsuke" sort="Baba, Shunsuke" uniqKey="Baba S" first="Shunsuke" last="Baba">Shunsuke Baba</name><name sortKey="Furuichi, Yasushi" sort="Furuichi, Yasushi" uniqKey="Furuichi Y" first="Yasushi" last="Furuichi">Yasushi Furuichi</name><name sortKey="Hara, Kenji" sort="Hara, Kenji" uniqKey="Hara K" first="Kenji" last="Hara">Kenji Hara</name><name sortKey="Ito, Kenji" sort="Ito, Kenji" uniqKey="Ito K" first="Kenji" last="Ito">Kenji Ito</name><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><name sortKey="Nagasaka, Tetsuro" sort="Nagasaka, Tetsuro" uniqKey="Nagasaka T" first="Tetsuro" last="Nagasaka">Tetsuro Nagasaka</name><name sortKey="Nakamura, Sayaka" sort="Nakamura, Sayaka" uniqKey="Nakamura S" first="Sayaka" last="Nakamura">Sayaka Nakamura</name><name sortKey="Umemura, Eri" sort="Umemura, Eri" uniqKey="Umemura E" first="Eri" last="Umemura">Eri Umemura</name><name sortKey="Wakabayashi, Toshihiko" sort="Wakabayashi, Toshihiko" uniqKey="Wakabayashi T" first="Toshihiko" last="Wakabayashi">Toshihiko Wakabayashi</name><name sortKey="Wakabayashi, Toshihiko" sort="Wakabayashi, Toshihiko" uniqKey="Wakabayashi T" first="Toshihiko" last="Wakabayashi">Toshihiko Wakabayashi</name><name sortKey="Yamada, Yoichi" sort="Yamada, Yoichi" uniqKey="Yamada Y" first="Yoichi" last="Yamada">Yoichi Yamada</name></country><country name="États-Unis"><region name="Californie"><name sortKey="Yamada, Yoichi" sort="Yamada, Yoichi" uniqKey="Yamada Y" first="Yoichi" last="Yamada">Yoichi Yamada</name></region><name sortKey="Klein, Ophir D" sort="Klein, Ophir D" uniqKey="Klein O" first="Ophir D." last="Klein">Ophir D. Klein</name><name sortKey="Nakamura, Sayaka" sort="Nakamura, Sayaka" uniqKey="Nakamura S" first="Sayaka" last="Nakamura">Sayaka Nakamura</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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